Our study aimed to obtain lysate from a lactic acid bacteria (LAB) S. macedonicus MBF 10-2 through an optimized fermentation on plant based-medium using the Response Surface Methodology. Lactic acid bacteria (LAB) could serve as potential candidates for bioactive lysate; the lactic acids have been utilized for their moisturizing, antimicrobial, and rejuvenating effects, while other substances provide topical benefits and health effects for the skin. In this study, strain MBF10-2 was grown in a fermenter tank in plant based-modified de Man Rogosa and Sharpe (MRS) medium, i.e. Soy-peptone and Vegitone. These plant-based peptones were used in order to promote Muslim’s restriction for non-halal materials, as well as to support other groups of people who have vegan or vegetarian preferences. The duration and the medium composition were optimized to obtain the best production condition of cell lysate. Central Composite Design was employed for Design Expert 7.0.0 by adjusting 3 factors: dextrose, soy or proteose peptone, and duration of fermentation. Bacteriocin-Like Inhibitory Substance (BLIS) activity and pH of lysate were used as indicators. We found that the optimum condition for lysate production using MRS Soy Peptone and Vegitone are, i.e: dextrose concentration 2.5%, plant-based peptone 1.25%, while optimum fermentation durations were 11.18 hrs (MRS Soy Peptone) and 17 hrs (MRS Vegitone). However, based on BLIS activity and pH of the lysate, standard MRS medium produced better quality lysate.
Laboratory of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy-Universitas Indonesia (1990-present)
E-mail : amarila.malik@ui.ac.id
• Major research interests: microbiology, biotechnology, molecular biology, genetic engineering, bio-prospect of microbes, and pharmacogenomic/pharmacogenetic, as reflected in her publications.
• Bachelor degree on Pharmacy and professional Pharmacist from Universitas Indonesia (1989), Master degree on Pharmacy (natural product pharmacy) from Bandung Institute of Technology, Bandung, Indonesia (1993), Doctoral degree on Biology (Microbiology) from Bogor Agriculture Institute, Bogor, Indonesia (2000).
• Postdoctoral research associate of molecular biology research at the Department of Biological Sciences, Oakland University, Michigan, USA (2003) and postdoctoral research fellow of microbiology and molecular genetics research at the Department of Microbiology and Molecular Genetics, The University of Texas-Health Science Center at Houston, Houston, Texas, USA (2004).
• Research training, visits and collaborations with world scientists, i.e. the Netherlands (Rijks-universiteit Groningen, Groningen), Japan (Nara Institute of Science and Technology-NAIST, Nara), New Zealand (University of Otago, Dunedin), Vietnam (Vietnam National University-VNU/Institute of Microbiology and Biotechnology-IMBT, Hanoi).
• Received research grants/fellowships from Indonesia and abroad (e.g. SPIN-KNAW, TWAS, Italy).
• In 2010 received the Best Research Award winner of the most prestigious research award in Indonesia, Ristek Kalbe Science Award (RKSA).
2014-2019 Faculty of Pharmacy-Universitas Indonesia(student)
2019-present PT Taisho Pharmaceutical Indonesia Tbk.
E-mail : dini.andyanti@alumni.ui.ac.id
Microbiology Laboratory Assistant, Faculty of Pharmacy, Universitas Indonesia
Biotechnology Laboratory Assistant, Faculty of Pharmacy, Universitas Indonesia
Bachelor of Pharmacy, Universitas Indonesia
Pharmacist, Universitas Indonesia
Microbiology Laboratory Assistant, Faculty of Pharmacy, Universitas Indonesia
Resveratrol Extract Metabolit Anti-Syndrome Granule from Gnetum Gnemon Seeds with NADES Solvent (Tanoto Student Research Award, Indonesia)
Expression & Purification of T brucei Putative RNA Methyltransferase Involved in Cap 4 Formation (9th Summer Research Program 2018, University of Tsukuba)
Production of Streptococcus macedonicus MBF10-2 Lysate Using Plant-based de Man, Rogosa and Sharpe Modified Medium Performing Response Surface Methodology (Microbiology and Biotechnology Letter, 2019)
The Laboratory of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, Universitas Indonesia integrates multi-disciplines, i.e. microbiology, genetics, and biotechnology, that form the basis and applied research in understanding the important of molecular genetics and genetic engineering, bioconversion and bioprocess, pharmacy and clinical microbiology, as well as genome and proteome studies (microbes, mammalian based) and transfer them to the industrial aspect in food, pharmaceutical and cosmetic industries, with various and innovative methods.
We have published quality papers in high profile scientific journals and produced patents as generated by researches conducted by excellent undergraduate and graduate students, as well as young scientists. The labs are funded by competitive grants from both national and international sources. Collaborations have been established with various research institutes and laboratories in Europe, Asian, and New Zealand.
Current research project
- Exploitation of antimicrobial peptide from lactic acid bacteria
- Bio-prospect of microbiota from environment, food, and human samples
- Development of probiotic and postbiotic for microbiome therapeutics
- Pharmacogenomic studies of clinical samples as a translational research approach to study degenerative diseases
Photo of Laboratory of Pharmaceutical Microbiology and Biotechnology,
Faculty of Pharmacy-Universitas Indonesia
