Univ. of California, San Francisco (UCSF), 박사후연구원/전문연구원
2019 ~ 2020
종근당 효종연구소, 책임연구원
2020 ~ 현재
한국생명공학연구원 바이오신약중개연구센터, 선임연구원
주요 연구 관심 분야 소개
항체 라이브러리 제작 및 신규 항체 개발
혈관질환/암 치료용 항체, 이중항체, 항체-단백질 복합체 개발
항암 항체-약물 접합체 개발
연구성과 - 주요 논문
1. Park J, Lee DG, Lee NG, Kwon MG, Son YS, Son MY, Bae KH, Lee J, Park JG, Lee NK*, Min JK*. Monoclonal antibody K312-based depletion of pluripotent cells from differentiated stem cell progeny prevents teratoma formation. 2022. BMB Reports. 55(3):142-147. *corresponding author
2. Lee NK, Oh SH, Choi YJ, Kang SK. Identification of a peptide ligand facilitating percutaneous delivery of macromolecular cargo via inner root sheath in hair follicles by phage display combined with quantitative real-time PCR as a validation tool. 2022. J Drug Deliv Sci Tech. 67:102965.
3. Kim JH, Lee C, Jeon HJ, Kim BK, Lee NK, Choi SK, Han JE. First report on Enterocytozoon hepatopenaei (EHP) infection in Pacific white shrimp (Penaeus vannamei) cultured in Korea. 2021. Aquaculture. 547: 737525-737525.
4. Oh SH, Kim SH, Jeon JH, Kim EB, Lee NK, Beck S, Choi YJ, Kang SK. Cytoplasmic expression of a model antigen with M Cell-Targeting moiety in lactic acid bacteria and implication of the mechanism as a mucosal vaccine via oral route. 2021. Vaccine. 39(30):4072-4081.
5. Su Y, Bidlingmaier S, Behrens C, Lee NK, Liu B. ALPPL2 is a highly specific and targetable tumor cell surface antigen. 2020. Cancer Res. DOI: 10.1158/0008-5472.CAN-20-1418.
6. Lee NK, Su Y, Bidlingmaier S, Liu B. Manipulation of cell-type selective antibody internalization by a guide-effector bispecific design. 2019. Mol. Cancer Ther. doi: 10.1158/1535-7163.MCT-18-1313.
7. Su Y*, Liu Y*, Behrens C*, Bidlingmaier S*, Lee NK, Aggarwal R, Sherbenou D, Burlingame A, Hann B, Simko J, Premasekharan G, Paris P, Shuman MA, Seo Y, Small E, Liu B. Targeting CD46 for both adenocarcinoma and neuroendocrine prostate cancer. 2018. JCI Insight. doi: 10.1172/jci.insight.121497.
8. Lee NK*, Zhang Y*, Su Y, Bidlingmaier S, Sherbenou D, Ha KD, Liu B. Cell-type specific potent Wnt signaling blockade by bispecific antibody. 2018. Sci. Rep. 15;8(1):766.
9. Lee NK, Bidlingmaier S, Su Y, Liu B. Modular construction of large non-immune human antibody phage display libraries from variable heavy and light chain gene cassettes. 2018. Method Mol. Biol. 1701(4), 61-82.
10. Su Y, Bidlingmaier S, Lee NK, Liu B. Combine phage antibody display library selection on patient tissue specimens with laser capture microdissection to identify novel human antibodies targeting clinically relevant tumor antigens. 2018. Method Mol. Biol. 1701(21), 331-347.
11. Ha KD, Bidlingmaier S, Su Y, Lee NK, Liu B. Identification of novel macropinocytosing human antibodies by phage display and high-content analysis. 2016. Method Enzymol. 585(7), 91-110.
12. Bidlingmaier S, Ha KD, Lee NK, Su Y, Liu B. Proteome-wide identification of novel ceramide-binding proteins by yeast surface cDNA display and deep sequencing. 2016. Mol. Cell. Proteomics. 15(4), 1232-1245.
13. Lee KW*, Lee NK*, Ham S, Roh TY, Kim SH. Twist1 is essential in maintaining mesenchymal state and tumor-initiating properties in synovial sarcoma. 2014. Cancer Lett. 343, 62-73. *Equal contribution
14. Ha SY, Kim KY, Lee NK, Kim MG, Kim SH. Overexpression of matriptase correlates with poor prognosis in esophageal squamous cell carcinoma. 2014. Virchows Arch. 464, 19-27.
15. Jung E, Lee NK, Kang SK, Choi SH, Kim D, Park K, Choi K, Choi YJ, Jung DH. Identification of tissue-specific targeting peptide. 2012. J. Comput. Aided. Mol. Des. 26, 1267-1275.
16. Lee KW*, Lee NK*, Kim JH, Kang MS, Yoo HY, Kim HH, Um SH, Kim SH. Twist1 directly causes the transcriptional repression of claudin-4 with prognostic significance in esophageal cancer. 2012. Biochem. Biophy. Res. Commun. 423, 454-460. *Equal contribution
17. Lee NK*, Kim HS*, Kim KH, Beck S, Kim EB, Cho CS, Kang SK, Choi YJ. Identification of a novel peptide ligand targeting visceral adipose tissue via transdermal route by in vivo phage display. 2011. J. Drug Target. 19, 805-813.
18. Beck S, Jin X, Yin JL, Kim SH, Lee NK, Oh SY, Jin X, Kim MK, Kim EB, Son JS, Kim SC, Nam DH, Kim SH, Kang SK, Kim HG, Choi YJ. Identification of a peptide that interacts with Nestin protein expressed in brain cancer stem cells. 2011. Biomaterials. 32, 8518-8528.
19. Jung E, Choi SH, Lee NK, Kang SK, Choi YJ, Shin JM, Choi KH, Jung DH. Machine Learning Study for the Prediction of Transdermal Peptide. 2011. J. Comput. Aided. Mol. Des. 25, 339-347.
20. Zhang QK, Lee HG, Han JA, Kang SK, Lee NK, Baik MG, Choi YJ. Differentially expressed proteins associated with myogenesis and adipogenesis in skeletal muscle and adipose tissue between bulls and steers. 2011. Mol. Biol. Rep. DOI 10.1007/s11033-011-0821-3.
21. Lee NK, Kim MK, Choi JH, Kim EB, Lee HG, Kang SK, Choi YJ. Identification of a peptide sequence targeting mammary vasculature via RPLP0 during lactation. 2010. Peptides. 31, 2247-54.
22. Yoo MK, Kang SK, Choi JH, Park IK, Na HS, Lee HC, Kim EB, Lee NK, Nah JW, Choi YJ, Cho CS. Targeted delivery of chitosan nanoparticles to Peyer's patch using M cell-homing peptide selected by phage display technique. 2010. Biomaterials. 31, 7738-7747.
23. Jung E, Kim J, Choi SH, Kim M, Rhee H, Shin JM, Choi K, Kang SK, Lee NK, Choi YJ, Jung DH. Artificial Neural Network Study On Organ-Targeting Peptides. 2010. J. Comput. Aided. Mol. Des. 24, 49-56.
24. Kim EB, Son JS, Jang QK, Lee NK, Kim SH, Choi JH, Kang SK, Choi YJ. Generation and characterization of thymidine/D-alanine auxotrophic recombinant Lactococcus lactis subsp. lactis IL1403 expressing BmpB. 2009. Curr. Microbiol. 61, 29-36.
25. Kang SK, Woo JH, Kim MK, Woo SS, Choi JH, Lee HG, Lee NK, Choi YJ. Identification of a peptide sequence that improves transport of macromolecules across the intestinal mucosal barrier targeting goblet cells. 2008. J. Biotechnol. 135, 210-216.
26. Jin X, Kim JG, Oh MJ, Oh HY, Sohn YW, Pian X, Yin JL, Beck S, Lee NK, Son JS, Kim HG, Yan CG, Wang JH, Choi YJ, Whang KY. Opposite roles of MRF4 and MyoD in cell proliferation and myogenic differentiation. 2007. Biochem. Biophy. Res. Commun. 364, 476-482.
연구성과 - 주요특허
1. Lee J, Lee NK, et al., Novel anti-SEB antibodies and uses thereof. 2021_submitted. 10-2021-0134030.
2. Liu B, Lee NK, Su Y, Bidlingmaier S. Compositions and methods for modulating cellular internalization. 2020_published. WO/2020/180398.
3. Liu B, Lee NK, Su Y, Bidlingmaier S. CD38 and ICAM1 antibodies and uses thereof. 2020_published. PCT/US19/61884.
4. Liu B, Lee NK, Su Y, Bidlingmaier S. Engineered antibodies and uses thereof. 2018_published. PCT/US2017/062418.
5. Liu B, Su Y, Bidlingmaier S, Lee NK, Behrens C. Macropinocytosing human anti-CD46 antibodies and targeted cancer therapeutics. 2020_granted. US10533056 B2.
6. Kim SH, Nam DH, Lee NK, Park HK, Joo KM. Antibodies cross-reactive to human and mouse c-Met and uses thereof. 2016_granted. US9296817 B2.
7. Nam DH, Kim SH, Yoon Y, Lee NK, Joo KM, Park HK. Single-chain Fv antibody library, method for preparing thereof and screening method of single-chain Fv antibody by using the same. 2015_published. WO2015133882 A1.
8. Choi YJ, Beck S, Kang SK, Lee NK, Jin X. A peptide binding undifferentiated glioma stem cells. 2014_granted. South Korea. KR101356740B1.
9. Choi YJ, Kang SK, Lee NK, Beck S. Transdermal peptides traversing across hair follicles in the skin. 2013_granted. South Korea. KR101327555B1.
