논문 요약

L-malic acid (L-MA) plays an increasingly important role and is gaining great interest in various industries as it can be used in a wide variety of foods and non-foods products. However, few microorganisms have been able to efficiently produce L-MA from xylose derived from lignocellulosic biomass (LB). To overcome this problem, we promote new technologies to facilitate the bioconversion of LB into fermentable sugars without pretreatment, which not only reduces costs but also eliminates unwanted compounds during the pretreatment process. In this research, the unique trifunctional xylanolytic in-house enzyme, PcAxy43A from Paenibacillus curdlanolyticus B-6 was selected to hydrolyze xylan in untreated LB, especially corn hull to xylose, in one step. Moreover, we found that the newly isolated mesophilic bacterium, Acetobacter tropicalis strain H1 (biosafety level 1) was able to produce L-MA from xylose with a high L-MA titer of 77.09 g/l, with a yield of 0.77 g/g and a productivity of 0.64 g/l/h from the xylose derived from corn hull. Our findings demonstrates a good strategy to present a good method for producing L-MA from LB, which is an attractive, economical, and environmentally friendly biological process.

교신저자

제1저자

연구실 소개

At Excellent center for enzyme and microbial utilization, Pilot Plant Development and Training instituted and School of Bioresources Technology, King Mongkut’s university of technology Thonburi. Our expert members including of Assoc. Prof. Dr. Khanok Ratanakhanokchai (Head of the excellent center), Assoc. Prof. Dr. Chakrit Tachaapaikoon (Head of Enzyme technology Laboratory) and Asst. Prof. Dr. Rattiya Waeonukul. We takes it upon ourself to provide exceptional research and service to motivate new Bio-Circular-Green Economic Model or BCG project, promoted by the Thai government as a new economic model for inclusive and sustainable growth. Over the past our team created an impact research projects which uses agricultural waste to create new products and has promoted the use of different biological resources in each region to gain the most benefit for local communities. To screening and elucidation of effective novel bacterial and multienzyme complex system for bio-conversion of agricultural waste to high value added products. Emphasis of environmental and green technology, more concern for that issue and develop our enzyme and microbial technology to produce high value from agricultural residue, to eliminate burning, effecting on air pollution.