University of Michigan, Howard Hughes Medical Institute, 박사후연구원
2021 ~
현재 아주대학교 생명과학과, 조교수
주요 연구 관심 분야 소개
세균의 단백질 항상성 유지 시스템 연구
세균의 숙주 내 단백질 타겟 스트레스 반응 연구
단백질 항상성 개량 세균을 이용한 단백질 생산 플랫폼 개발
연구성과 - 주요 논문
Lee, C., Betschinger, P., Wu, K., Zyla, D., Glockshuber, R. & Bardwell, J. C. A. (2020) A metabolite binding protein moonlights as a bile-responsive chaperone. EMBO J. 3:e104231. doi: 10.15252/embj.2019104231
Lee, C., Franke, K. B., Kim, H., Kamal, S. M., Lünsdorf, H., Jäger, J., Nimtz, M., Trček, J., Jänsch, L., Bukau, B., Mogk, A. & Römling, U. (2018) The stand-alone ClpG disaggregase confers superior heat tolerance to bacteria. Proc Natl Acad Sci U S A 115(2):E273-E282. doi: 10.1073/pnas.1712051115
Wu, K., Stull, F., Lee, C. & Bardwell, J. C. A. (2019) Protein folding while chaperone bound is dependent on weak interactions. Nature Communications10(1):4833. doi: 10.1038/s41467-019-12774-6(F1000Prime recommended)
연구성과 - 전체 논문
* Equal contributions, # corresponding author
23. Kim, H.*, Wu, K.*, Lee, C.#. Stress-responsive periplasmic chaperones in bacteria. Front Mol Biosci. 2021 (in press)
22. Kamal, S. M., Cimdins-Ahne, A., Lee, C., Li, F., Martin Rodriguz, AJ., Sefebekova, Z., Afasizhev, R,, Wami, H., Katikaridis, P., Meins, L., Lünsdorf, H., Dorbrindt, U., Mogk, A., & Römling, U. (2021) A recently isolated human commensal Escherichia coli ST10 clone member mediates enhanced thermotolerance and tetrathionate respiration on a P1 phage derived IncY plasmid. Mol. Microbiol.2021 Feb 115(2):255-271. doi: 10.1111/mmi.14614.
21. Lee, C.*, Klockgether, J. *, Fischer, S. *, Trcek, J., Tummler, B. & Römling, Ute. (2020) Why? - Successful Pseudomonas aeruginosa clones with a focus on clone C. FEMS Microbiology Reviews doi: 10.1093/femsre/fuaa029 (*Equal contributions)
20. Ying, L., Lee, C., Fengyang, L., Trcek, J., Bähre, H., Guo, R., Chen, C., Chernobrovkin, A., Zubarev, R. & Römling, U. (2020) A cyclic di-GMP network is present in Gram-positive Streptococcus and Gram-negative Proteus species. ACS Infectious Diseases doi: 10.1021/acsinfecdis.0c00314
19.Lee, C., Betschinger, P., Wu, K., Zyla, D., Glockshuber, R. & Bardwell, J. C. A. (2020) A metabolite binding protein moonlights as a bile-responsive chaperone. EMBO J. 3:e104231. doi: 10.15252/embj.2019104231
18. Wu, K., Stull, F., Lee, C. & Bardwell, J. C. A. (2019) Protein folding while chaperone bound is dependent on weak interactions. Nature Communications10(1):4833. doi: 10.1038/s41467-019-12774-6(F1000Prime recommended)
17. Kamal, S. M., Rybtke, M. L., Nimtz, M., Sperlein, S., Giske, C., Trček, J., Deschamps, J., Briandet, R., Dini, L., Jänsch, L., Tolker-Nielsen, T., Lee, C., Römling, U. (2019) Two FtsH Proteases Contribute to Fitness and Adaptation of Pseudomonas aeruginosa Clone C Strains. Frontiers in Microbiology 10:1372.doi: 10.3389/fmicb.2019.01372
16.Lee, C. * †, Kamal, S. M. *, & Römling, U. † (2019) High frequency of double crossover recombination facilitates genome engineering in Pseudomonas aeruginosa PA14 and clone C strains. Microbiology165(7):757-760. doi: 10.1099/mic.0.000812
15. Lee, C., Kim, H., & Bardwell, J. C. A. (2018) Electrostatic interactions are important for chaperone-client interaction in vivo. Microbiology 164(7):992-997. doi: 10.1099/mic.0.000676
14. Lee, C., Franke, K. B., Kim, H., Kamal, S. M., Lünsdorf, H., Jäger, J., Nimtz, M., Trček, J., Jänsch, L., Bukau, B., Mogk, A. & Römling, U. (2018) The stand-alone ClpG disaggregase confers superior heat tolerance to bacteria. Proc Natl Acad Sci U S A 115(2):E273-E282. doi: 10.1073/pnas.1712051115
13. Lee, C. & Park, C. (2017) Bacterial Responses to Glyoxal and Methylglyoxal: Reactive Electrophilic Species. Int J Mol Sci. 18(1).pii: E169. doi: 10.3390/ijms18010169
12. Lee, C., Kim, J., Kwon, M., Lee, K., Min, H., Kim, S.H., Kim, D., Lee, N., Kim, J., Kim, D., Ko, C. & Park, C. (2016) Screening for Escherichia coli K-12 genes conferring glyoxal resistance or sensitivity by transposon insertions. FEMS Microbiol Lett. 363(18).pii: fnw199. doi: 10.1093/femsle/fnw199
11. Lee, C. †, Wigren, E., Lünsdorf, H. & Römling, U. † (2016) Protein homeostasis - more than resisting a hot bath. Curr Opin Microbiol. 30:147-154. doi: 10.1016/j.mib.2016.02.006
10. Lee, C. *, Lee, J. *, Lee, J., & Park, C., (2016) Characterization of the Escherichia coli YajL, YhbO, and ElbB glyoxalases. FEMS Microbiol Lett. 363(3). pii: fnv239. doi: 10.1093/femsle/fnv239(*Equal contributions)
9. Lee, C., Wigren, E., Trček, J., Peters, V., Kim, J., Hasni, H., Nimtz, M., Lindqvist, Y., Park, C., Curth, U., Lünsdorf, H. & Römling, U. (2015) A novel protein quality control mechanism contributes to heat shock resistance of world-wide distributed Pseudomonas aeruginosa clone C strains. Environ. Microbiol. 17(11):4511-26. doi: 10.1111/1462-2920.12915
8. Lee, C., Peters, V., Melefors, O. & Römling U. (2014) Draft Genome Sequence of Pseudomonas aeruginosa SG17M, an Environmental Isolate Belonging to Clone C, Prevalent in Patients and Aquatic Habitats. Genome announcements. 20;2(2). pii: e00186-14. doi: 10.1128/genomeA.00186-14
7. Lee, C., Kim, I. & Park, C. (2013) Glyoxal detoxification in Escherichia coli K-12 by NADPH dependent aldo-keto reductases. J Microbiol. 51(4):527-30. doi: 10.1007/s12275-013-3087-8
6. Lee, C., Shin. J. & Park, C. (2013) Novel regulatory system nemRA-gloA for electrophile reduction in Escherichia coli K-12. Mol. Microbiol. 88(2):395-412. doi: 10.1111/mmi.12192
5.Lee, C.& Park, C. (2013) Mutation Upregulating the flhDC operon of Escherichia coli K-12. J Microbiol. 51(1):140-4. doi: 10.1007/s12275-013-2212-z
4.Lee, C. & Park, C. (2012) Development of a suicidal vector-cloning system based on butanal susceptibility due to an expression of YqhD aldehyde reductase. J Microbiol. 50(2):249-55. doi: 10.1007/s12275-012-1438-5
3. Kwon, M., Lee, J., Lee, C. & Park, C. (2012) Genomic rearrangements overexpressing an aldo-keto reductase YafB of Escherichia coli confers resistance to glyoxal. J Bacteriol. 194(8):1979-88. doi: 10.1128/JB.06062-11
2.Lee, C., Kim, I., Lee, J., Lee, K. L., Min, B. & Park, C. (2010) Transcriptional activation of the aldehyde reductase YqhD by YqhC and its implication in glyoxal metabolism of Escherichia coli K-12. J Bacteriol 192(16):4205-14. doi: 10.1128/JB.01127-09
1. Kim, I., Kim, J., Min, B., Lee, C. & Park, C. (2007) Screening of genes related to methylglyoxal susceptibility. J Microbiol 45, 339-43
