Continued growth in the probiotic market has resulted in a widening of the product range. This has results in both increased consumer expectations but also a tightening of regulatory measures. Defined to be live microorganisms, probiotics should provide a benefit to the host when consumed at a proper dose and in a viable state. Procedures for maintaining the shelf-life of probiotic cultures may damage the integrity of the cells due to stress conditions during both the freezing and (vacuum-) drying process, thereby negatively influencing their functionality. We propose a lysine-based mixture for rehydration of freeze-dried probiotics for improving their viability during in vitro simulated gastric and duodenum stress conditions. Measurement of the zeta potential served as an indicator of cell integrity and efficacy of this mixture, while functionality was estimated by adhesion to a human enterocyte-like Caco-2 cell-line. The freeze-dried bacteria exhibited a significantly different zeta potential compared to fresh cultures; however, this condition could be restored by rehydration with the lysine mixture. Recovery of the surface charge was found to influence adhesion ability to the Caco-2 cell-line. The lysine Zeta-bio formulation also notably increased the adherence ability of lyophilized Lp-115 to the Caco-2 cell-line after subjected to in vitro stress conditions simulating the gastrointestinal tract passage.
Laboratory of Human Effective Microbes, Department of Advanced Convergence and HEM Pharma Inc., Business Incubator Center 103, Handong Global University
E-mail : wilhelm@woodapple.net
Dr. rer. nat. at the Technical University of Munich, Germany
Former Head of the Federal Research Institute of Hygiene and Toxicology at the BFE, Karlsruhe, now Max Rubner Institute, Germany
Honorary Professor at Karlsruhe Technical University (KIT), Germany
President of the ICFMH of the IUMS
Member of IUMS Subcommittee on the Taxonomy of Bifidobacterium and Lactobacillus and related organisms
Full Professor at Handong Global University, South Korea
Chair Professor at Handong Global University, South Korea
>360 publications in scientific journals; >70 book chapters; editor of 8 scientific textbooks.
Laboratory of Human Effective Microbes, Department of Advanced Convergence, Handong Global University
E-mail : karay.mx@gmail.com
Bachelor’s Degree at University of Guadalajara, Mexico
Master’s in Sciences in Biotechnological Processes at University of Gudalajara, Mexico
Ph.D. in Microbiology at Advanced Convergence, Handong Global University, South Korea
Technical Assistant at BIOZOO S.A de C.V., Mexico
Technical Assistant at CIAJ Laboratory, Mexico
High School Teacher at Monterrey Institute of Technology and Higher Education, Mexico
8 publications in scientific journals
HEM Pharma Inc., Business Incubator Center 103, Handong Global University
E-mail : hrpark@hempharma.bio
Bachelor’s Degree at Handong Global University, South Korea
Master’s in Microbiology in Laboratory of Human Effective Microbes at Handong Global University, South Korea
Ph.D. in Microbiology in Laboratory of Human Effective Microbes at Handong Global University, South Korea
Research Scientist at HEM Pharma Inc., South Korea
16 papers in scientific journals
The Laboratory of Human Effective Microbes at Handong Global University together with HEM Pharma Inc. studies the modulatory effect of probiotics on the host, with special focus on the host microbiome in disease states such as obesity, diabetes, hypercholesterolemia, inflammatory bowel disease, asthma, and depression. HEM Pharma Inc. is a research-based bio-company, initiated as start-up at Handong Global University, that aims to provide a personalized therapeutic solution through investigating of the impact of our microbiome and its metabolites on our overall health.
