신진과학자 소개

박 희 호 (Hee Ho Park)

강원대학교 화학생물공학부 생물공학전공 E-mail : hhpark@kangwon.ac.kr

주요 연구 관심 분야 소개

• 항체(CHO 동물세포 발현), 제약 단백질(미생물 발현) 및 단백질 나노입자 생성 등 고부가가치 화장품, 의약품 개발 연구
• 직접교차분화(Direct reprogramming / Transdifferentiation)를 통한 유도신경세포, 유도심근세포, 유도근육세포 생성 연구
• 유전자 가위(CRISPR) 및 바이러스(Lenti virus, Gammaretro virus)를 이용한 유전자 편집/재조합
• 면역세포치료제(CAR T, CAR NK) 개발
• 미세먼지/미세플라스틱에 의한 독성 및 분화 분석
• 인간 만능줄기세포(Human iPSC)를 이용한 오가노이드 개발
• 소 배양육(인공육) 개발

연구성과 - 논문

• 1. Kwon, M., Park, H. H.* (2020) " CAR T therapy targeting solid tumor", KSBB Journal, Accepted
• 2. Hong, S., Park, H. H.* (2020) "Drug delivery system using protein nanoparticles", KSBB Journal, Accepted
• 3. Ryu, J., Hwang, N. S., Park, H. H.*, Park, T. H.* (2020) "Protein-based direct reprogramming of fibroblasts to neuronal lineage cells", The International Journal of Biochemistry & Cell Biology, 121, 105717
• ^* Co-corresponding author
• 4. Lee, J., Park, H. H., Lim, K. S., Lee, H. J., Ha, S. J.* (2019) "A case study on biotechnology-related curriculum linked extracurricular program - focusing on K university CUBE program", KSBB Journal, 34, 4, 359-365
• 5. Lee, J., Park, H. H., Park, J. H.* (2019) "Efficient production of cell-permeable Oct4 protein using silkworm-originating 30Kc19 protein" Biotechnology and Bioprocess Engineering, 24, 6, 1-8
• 6. Ryu, J., Park, S. W., Park, H. H.*, Park, T. H.* (2019) "Enhanced single-cell viability using 30Kc6 for efficient expansion of human induced pluripotent stem cells", Process Biochemistry, 78, 161–168
• ^* Co-corresponding author
• 7. Razzak, A., Lee, J. E., Park, H. H., Park, T. H., Choi, S. S.* (2018) "Exploring binding mechanisms between curcumin and silkworm 30Kc19 protein using spectroscopic analyses and computational simulations", Biotechnology and Bioprocess Engineering, 5, 23, 605-616
• 8. Park, H. H., Triboulet, R., Bentler, M., Guda, S., Du, P., Xu, H., Gregory, R. I., Brendel, C., Williams, D. A.* (2018) "DROSHA knockout leads to enhancement of viral titers for vectors encoding miRNA adapted shRNAs", Molecular Therapy-Nucleic Acids, 12, 591-599
• 9. Park, H. H.1, Woo, W. H.1, Ryu, J., Lee, H. J., Park, J. H.*, Park, T. H.* (2017) "Enzyme delivery using protein-stabilizing and cell-penetrating 30Kc19α protein nanoparticles", Process Biochemistry, 63, 76-83
• 10. Han, U.1, Park, H. H.1, Kim Y. J. Park, T. H., Park, J. H.*, Hong, J.* (2017) "Efficient encapsulation and sustained release of basic fibroblast growth factor in nanofilm: extension of the feeding cycle of human induced pluripotent stem cell culture", ACS Applied Materials and Interfaces, 9, 30, 25087-25097
• ¹ Co-first author
• 11. Choi, M.1, Park, H. H.1, Choi, D., Han, U., Park, T. H., Lee, H., Park, J. H., Hong, J.* (2017) "Multilayer nanofilms via inkjet printing for stabilizing growth factor and designing desired cell developments", Advanced Healthcare Materials, 6, 14, 1700216
• ¹ Co-first author
• 12. Ryu, J., Kim, H., Park. H. H., Park, J. H., Lee, H. J., Rhee, W. J., Park, T. H.* (2016) "Protein‐stabilizing and cell‐penetrating properties of α‐helix domain of 30Kc19 protein", Biotechnology Journal, 11, 1443-1451
• 13. Lee, H. J.1, Park, H. H.1, Sohn, Y., Park, J. H., Ryu, J., Rhee, W. J., Park, T. H.* (2016) "Alpha-galactosidase delivery using 30Kc19-human serum albumin nanoparticles for effective treatment of Fabry disease", Applied Microbiology and Biotechnology, 100, 24, 10395-10402
• ¹ Co-first author
• 14. Park, H. H.1, Choi, J.1, Lee, H. J., Park, J. H., Ryu, J., Rhee, W. J.*, Park, T. H.* (2015) "Enhancement of human erythropoietin production in Chinese hamster ovary cells through supplementation of 30Kc19-30Kc6 fusion protein", Process Biochemistry, 50, 6, 973-980
• 15. Ryu, J., Park. H. H., Park, J. H., Lee, H. J., Rhee, W. J., Park, T. H.* (2016) "Soluble expression and stability enhancement of transcription factors using 30Kc19 cell-penetrating protein", Applied Microbiology and Biotechnology, 100, 3523-3532
• 16. Park, H. H., Sohn, Y., Yeo, J. W., Park, J. H., Lee, H. J., Ryu, J., Rhee, W. J., Park, T. H.* (2014) "Dimerization of 30Kc19 protein in the presence of amphiphilic moiety and importance of Cys-57 during cell penetration", Biotechnology Journal, 9, 1582-1593
• 17.Park, J. H., Lee, H. J., Park, H. H., Rhee, W. J., Park T. H.* (2014) "Stabilization of cellular mitochondrial enzyme complex and sialyltransferase activity through supplementation of 30Kc19 protein", Applied Microbiology and Biotechnology, 99, 2155-2163
• 18. Park, H. H., Sohn, Y., Yeo, J. W., Park, J. H., Lee, H. J., Ryu, J., Rhee, W. J.*, Park, T. H.* (2014) "Identification and characterization of a novel cell-penetrating peptide of 30Kc19 protein derived from Bombyx mori", Process Biochemistry, 49, 1516-1526
• 19. Lee, H. J., Park, H. H., Kim, J. A., Park, J. H., Ryu, J., Choi, J., Lee, J., Rhee, W. J., Park, T. H.* (2014) "Enzyme delivery using the 30Kc19 protein and human serum albumin nanoparticles", Biomaterials, 35, 1696-1704
• 20. Lee, S. H., Rhee, H. -W., Van Noort, D., Lee, H. J., Park, H. H., Shin, I. -S., Hong, J. -I.*, Park, T. H.* (2014) "Microfluidic bead-based sensing platform for monitoring kinase activity", Biosensors and Bioelectronics, 57, 1-9
• 21. Park, J. H., Park, H. H., Choi, S. S., Park, T. H.* (2012) "Stabilization of enzymes by the recombinant 30Kc19 protein", Process Biochemistry, 47, 164-169
• 22. Park, J. H., Lee, J. H., Park, H. H., Rhee, W. J., Choi, S. S.*, Park, T. H.* (2012) "A protein delivery system using 30Kc19 cell-penetrating protein originating from silkworm”, Biomaterials, 33, 9127-9134
• 23. Park, J. H., Wang, Z., Jeong, H. -J., Park, H. H., Kim, B. -G., Tan, W. -S., Choi, S. S., Park, T. H.* (2012) "Enhancement of recombinant human EPO production and glycosylation in serum-free suspension culture of CHO cells through expression and supplementation of 30Kc19", Applied Microbiology and Biotechnology, 96, 671-683
• 24. Wang, Z, Park, J. H., Park, H. H., Tan, W., Park, T. H.* (2011) "Enhancement of recombinant human EPO production and sialylation in Chinese hamster ovary cells through Bombyx mori 30Kc19 gene expression", Biotechnology and Bioengineering, 108, 1634-1642
• 25. Wang, Z, Park, J. H., Park, H. H., Tan, W., Park, T. H.* (2010) "Enhancement of therapeutic monoclonal antibody production in CHO cells using 30Kc6 gene" Process Biochemistry, 45(12), 1852-1856
• 26. Park, J. H., Park, H. H., Park, T. H.* (2010) "Cellular engineering for the high-level production of recombinant proteins in mammalian cell systems" PKorean Journal of Chemical Engineering, 27(4), 1042-1048
• 27. Koo, T. Y., Park, J. H., Park, H. H., Park, T. H.* (2009) "Beneficial effect of 30Kc6 gene expression on production of recombinant interferon-β in serum-free suspension culture of CHO cells" Process Biochemistry, 44(2), 146-153
• 28. Oh, J. S., Park, H. H., Park, T. H.* (2008) "Temperature management strategy for efficient gene expression in a thermally inducible Escherichia coil/bacteriophage system" Biotechnology. and Bioprocess Engineering., 13, 470-475

연구성과 - 특허등록

• 1. Park T. H., Kim H. Ryu J. Park H. H. "Cell-penetration and enzyme-stabilizing properties of α-helix domain of the 30Kc19 protein and cargo delivery system using the same" Korean Patent Application Number 10-2015-0001973 (2015. 1. 7) PCT Patent Application Number PCT/KR2015/004297 (2015. 4. 29)
• 2. Park T. H., Park H. H., Yeo J. W. "Cell-penetrating peptide" Kor. Patent Application Number 10-2013-0025087 (2013. 3. 8) Korean Patent Registration Number 10-1475744 (2014.12.17)
• 3. Han K.-B., Park T. H., Park H. H., Park J. H. "Health care food silkworm hemolymph extract and process for preparing thereof" Korean Patent Application Number 10-2009-0020463 (2009. 3. 10)