책임교수 소개

[학력]

• 2013

  서울대학교 협동과정 생물화학공학전공 박사 (석박통합)

• 2005

  전남대학교 응용화학공학부 학사

연구분야 소개

(1) 주요 연구분야

본 연구실은 단백질의 구조 분석, 분자동역학, 도킹등을 통해 효소의 안정성, 기질특이성, 활성을 개량하는 연구를 진행한다. 개량된 효소는 유용물질의 생산 및 전환에 이용되며 적용 분야는 매우 다양하다.

(2) 연구 주제

▶ 인공금속효소 (artificial Metalloenzyme, Arm) 연구

인공금속효소는 전이금속촉매의 빠른 반응성 및 비자연계 촉매반응과 효소의 선택성 가능 반응을 동시에 구현 한다. 전이금속복합체 탐색, scaffold 단백질 탐색, 금속과 단백질 조합방법 모색, 반응 스크리닝법 구축, 분자동역학을 이용한 구조 분석, 움직임 모식화, 반응 매커니즘 등의 연구를 진행한다. Random mutation 방법을 이용하여 인공금속효소의 라이브러리를 만들고 높은 선택성의 사이클로프로판화 반응을 스크리닝 할 수 있는 효율적인 플랫폼을 만든 연구를 수행하였고 실제 인공금속효소 움직임을 분석하는 연구를 수행중이다.

<인공금속효소의 연구 내용>

▶ Molecular modeling을 통한 효소 및 단백질 개량

Molecular modeling (Molecular dynamics, Molecular docking 등)을 통하여 효소 및 단백질의 디자인 전략을 구축 실제 in vitro에 적용하는 연구를 수행한다. 다양한 효소들에 대한 연구를 수행해 왔으며 필요시 Directed evolution, library screening등을 함께 수행한다.

<Molecular modeling을 이용한 기질 특이성, 활성, 안정성 개량>

대표 연구성과

▶ 논문

• 1. Sungmin Byun*, Hyun June Park*, Jeong Chan Joo, and Yong Hwan Kim, (2019) “Enzymatic Synthesis of D-pipecolic Acid by Engineering the Substrate Specificity of Trypanosoma cruzi Proline Racemase and Its Molecular Docking Study”, Biotechnology and Bioprocess Engineering 24, 215-222
• 2. Hao Yang*, Alan M Swartz*, Hyun June Park*, Poonam Srivastava, Ken Ellis-Guardiola, David M Upp, Gihoon Lee, Ketaki Belsare, Yifan Gu, Chen Zhang, Raymond E Moellering, and Jared C Lewis, (2018) “Evolving artificial metalloenzymes via random mutagenesis”, Nature Chemistry, 10, 318–324.
• 3. James T Payne, Paul H Butkovich, Yi-Fan Gu, Kyle N Kunze, Hyun June Park, Duosheng Wang, and Jared C Lewis, (2018) ”Enantioselective Desymmetrization of Methylenedianilines via Enzyme-Catalyzed Remote Halogenation”, Journal of the American Chemical Society, 140 (2), 546–549.
• 4. So Won Kim, Md Hasanuzzaman, Munju Cho, Nam Hyun Kim, Hye-Young Choi, Jung Woo Han, Hyun June Park, Ji Won Oh, and Jae-Gook Shin, (2017) “Role of 14-3-3 sigma in over-expression of P-gp by rifampin and paclitaxel stimulation through interaction with PXR”, Cellular signaling, 31, 124-134.
• 5. Ketaki D Belsare, Mary C Andorfer, Frida S Cardenas, Julia R Chael, Hyun June Park, and Jared C Lewis, (2017) “A Simple Combinatorial Codon Mutagenesis Method for Targeted Protein Engineering”, ACS synthetic biology, 6 (3), 416-420.
• 6. Byung Hoon Jo, Tae Yoon Park, Hyun June Park, Young Joo Yeon, Young Je Yoo and Hyung Joon Cha, (2016) “Engineering de novo disulfide bond in bacterial α-type carbonic anhydrase for thermostable carbon sequestration”, Scientific Reports, 6, Article number: 29322.
• 7. Mary C. Andorfer, Hyun June Park, Jaylie Vergara-Coll and Jared C. Lewis, (2016) “Directed evolution of RebH for catalyst-controlled halogenation of indole C–H bonds”, Chemical Science, 7 (6), 3720-3729.
• 8. Young Joo Yeon, Hyung-Yeon Park, Kyungmoon Park, Hyun June Park, and Young Je Yoo, (2016) “Structural Basis for the Substrate Specificity of 3-Hydroxybutyrate Dehydrogenase”, Biotechnology and Bioprocess Engineering, 21 (3), 364-372.
• 9. Seung Kee Moon, So Ra Park, Ami Park, Hyun Mi Oh, Hyun Jung Shin, Eun Ju Jeon, Seiwhan Kim, Hyun June Park, Young Joo Yeon, and Young Je Yoo, (2016) “Substitution of Heavy Complementarity Determining Region 3 (CDR-H3) Residues Can Synergistically Enhance Functional Activity of Antibody and Its Binding Affinity to HER2 Antigen”, Molecules and Cells, 39 (3), 217–228.
• 10. So Won Kim, Md. Hasanuzzaman, Munju Cho, Ye Rang Heo, Min-Jung Ryu, Na-Young Ha, Hyun June Park, Hyung-Yeon Park and Jae-Gook Shin, (2016) “Casein Kinase 2 (CK2)-mediated Phosphorylation of Hsp90β as a Novel Mechanism of Rifampin-induced MDR1 Expression”, The Journal of Biological Chemistry, 290, 17029-17040.
• 11. Camila Flor J. Yagonia, Hyun June Park, So Yeon Hong, Young Je Yoo, (2015) “Simultaneous improvements in the activity and stability of Candida antarctica lipase B through multiple-site mutagenesis”, Biotechnology and Bioprocess Engineering, 20, 218-224.
• 12. Hyun June Park, Kyungmoon Park, Yong Hwan Kim, and Young Je Yoo “Computational approach for designing thermostable Candida antarctica lipase B by molecular dynamic simulation”, (2014) “Computational approach for designing thermostable Candida antarctica lipase B by molecular dynamic simulation”, Journal of Biotechnology, 192, 66-70.
• 13. Young Joo Yeon, Hyun June Park, Hyung-Yeon Park and Young Je Yoo (2014) “Effect of His-tag location on the catalytic activity of 3-hydroxybutyrate dehydrogenase”, Biotechnology and Bioprocess Engineering, 19 (5), 798-802.
• 14. So Yeon Hong, Hyun June Park, and Young Je Yoo (2014) “Flexibility analysis of activity-enhanced mutants of bacteriophage T4 lysozyme”, Journal of Molecular Catalysis B, 106, 95~99.
• 15. Hong Seung Yun, Hyun June Park, Jeong Chan Joo, and Young Je Yoo (2013) “Thermostabilization of Bacillus subtilis lipase A by minimizing the structural deformation caused by packing enhancement”, Journal of Industrial Microbiology and Biotechnology, 40 (11), 1223-1229.
• 16. Hyun June Park, Kyungmoon Park, and Young Je Yoo (2013) “Understanding the effect of tert-butanol on Candida antarctica lipase B using molecular dynamics simulations”, Molecular Simulation, 39 (8), 653-659.
• 17. Hyun June Park, Jeong Chan Joo, Kyungmoon Park, Yong Hwan Kim, and Young Je Yoo (2013) “Prediction of the solvent affecting site and the computational design of stable Candida antarctica lipase B in a hydrophilic organic solvent”, Journal of Biotechnology, 163 (3), 346– 352.
• 18. Hyun June Park, Jeong Chan Joo, Kyungmoon Park, and Young Je Yoo (2012) “Stabilization of Candida antarctica lipase B in hydrophilic organic solvent by rational design of hydrogen bond”, Biotechnology and Bioprocess Engineering, 17 (4), 722-728.
• 19. Myung Joo Ko, Hyun June Park, So Yeon Hong, and Young Je Yoo (2012) “Continuous biodiesel production using in situ glycerol separation by membrane bioreactor system”, Bioprocess and Biosystems Engineering, 35 (1-2), 69-75

▶특허

1. 차형준, 박태윤, 박현준, 연영주, 조병훈, 유영제 (2015), "신규한 이황화결합을 가진, 개량된 고온 안정성 탄산무수화효소 및 이의 이용", 10-2015-0020224. (등록)